TABLE 2.
Primers and synthetic DNA sequences used in this study
| Oligonucleotide primer | Sequencea |
|---|---|
| 3821 Pxut | ccagtgaaaagttcttctcctttactcatatgtatatcTCCTTGTTGTTCTTGGTTGTTG |
| 3822 Pxut | atgcagctggcacgacaggtttcccgactggaaagcggCAGGACACGGCGCGTATTCTCC |
| 4127 Pxut-tdtomato | aacagccaagcttgcatgcctgcagactagtcggatccTTACTTGTACAGCTCGTCCATG |
| 4128 Pxut-tdtomato | caagaacaacaaccaagaacaacaaggagaattctttttATGGTGAGCAAGGGCGAGGAG |
| 4132 xcpQ | gccaaaacagccaagcttgcatgcctgcagactagtTTATTCCGTCTTCAGTTCGCGCAG |
| 4133 xcpQ | acaacaaccaagaacaacaaggagatataATGTCCCAACCTTTGCTCCGCGCCCTGTTTG |
| 4168 Pxut-MCS | ctactgccgccaggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgtatcaggctgaaaatcttctctcatccgccaaTTATTCTATTTACCAAGCTTGCATGCGACTAGTTGGATCCTTGGGTCGACCGAAGAATTCAAAAATCctccttgttgttcttggttgttgttcttgggcgtgtcccgatactagcaacggccctgtgcctgccgattacgaaaatcaccaacacgcagtgcgattttgcgtattgagattcgccctctgcgcgcctagtctgtgcagaccgcctctacggcaagaggcccggaacaagcctgaaaacaatgaaaaccgtaccgcctgttcaccgcatcgccctgttgttcaacggcagcaagatctatgaccgcggcatcatcagcggcatcggcaactacctgagcagcacgcgcgcgtcttgggacctgtttctggaagaggattttctctgccgcttgaaaggta |
a
Capital letters indicate sequence that directs priming, except for oligonucleotide 4168, where capital letters indicate the sequence that replaced GFP on pMQ578. Lowercase letters indicate sequence that directs recombination.