TABLE 1.
Constructed plasmids and generated strains in this study
| Plasmid or strain | Related genotype and feature(s)a |
|---|---|
| Constructed plasmids | |
| pET21a-FRT-aroGfbr | Carrying feedback-resistant DAHP synthase (D146N) gene from E. coli |
| pET21a-FRT-pheAfbr | Carrying feedback-resistant fused chorismate mutase P and prephenate dehydratase (S330P) gene from E. coli |
| pET21a-FRT-tyrAfbr | Carrying feedback-resistant fused chorismate mutase T and prephenate dehydrogenase (M53I and A354V) gene from E. coli |
| pET21a-FRT-tktA | Carrying transketolase 1 gene from E. coli |
| pET21a-FRT(-8TC)-tktA | Carrying transketolase 1 gene under weak T7 promoter variant |
| pET21a-FRT-ppsA | Carrying phosphoenolpyruvate synthase gene from E. coli |
| pET21d-FRT-aroA | Carrying 5-enolpyruvylshikimate-3-phosphate synthetase gene from E. coli |
| pET21a-FRT-aroB | Carrying 3-dehydroquinate synthase gene from E. coli |
| pET21a-FRT-aroC | Carrying chorismate synthase gene from E. coli |
| pET21a-FRT-aroD | Carrying 3-dehydroquinate dehydratase gene from E. coli |
| pET21a-FRT-aroE | Carrying dehydroshikimate reductase gene from E. coli |
| pET21a-FRT-aroL | Carrying shikimate kinase II gene from E. coli |
| pET21a-FRT-aroALC | Carrying aroALC artificial operon |
| pET21a-FRT-aroEDB | Carrying aroEDB artificial operon |
| pET21a-FRT-aroLC | Carrying aroLC artificial operon |
| pET21a-FRT-aroAC | Carrying aroAC artificial operon |
| pET21a-FRT-aroAL | Carrying aroAL artificial operon |
| pET21a-FRT-aroDB | Carrying aroDB artificial operon |
| pET21a-FRT-aroEB | Carrying aroEB artificial operon |
| pET21a-FRT-aroED | Carrying aroED artificial operon |
| LacZ-reporter assay strains [derived from BW25113(DE3)] | |
| AR-G161 | adhE::FRT-Km-FRT-PT7lac-lacZ |
| AR-G162 | ldhA::FRT-Km-FRT-PT7lac-lacZ |
| AR-G163 | pflDC::FRT-Km-FRT-PT7lac-lacZ |
| AR-G164 | pykA::FRT-Km-FRT-PT7lac-lacZ |
| AR-G165 | ascF::FRT-Km-FRT-PT7lac-lacZ |
| Donor strain for P1 transduction [derived from BW25113(DE3)] | |
| AR-G60 | pflDC::FRT-Km-FRT |
| Donor strains for P1 transduction [derived from MG1655(DE3)] | |
| M-ARG3 | tyrR::FRT-Km-FRT-PT7lac-aroGfbr |
| M-ARG4 | ldhA::FRT-Km-FRT-PT7lac-pheAfbr |
| M-ARG5 | ldhA::FRT-Km-FRT-PT7lac-tyrAfbr |
| M-ARG7 | pykF::FRT-Km-FRT-PT7lac-aroA |
| M-ARG10 | adhE::FRT-Km-FRT-PT7lac-ppsA |
| M-ARG11 | pflBA::FRT-Km-FRT-PT7lac-tktA |
| M-ARG14 | pflDC::FRT-Km-FRT-PT7lac-tktA |
| M-ARG36 | pflDC::FRT-Km-FRT-PT7lac(-8TC)-tktA |
| M-ARG43 | pykF::FRT-Km-FRT-PT7lac-aroALC |
| M-ARG45 | ascF::FRT-Km-FRT-PT7lac-aroEDB |
| M-ARG72 | pykF::FRT-Km-FRT-PT7lac-aroAL |
| M-ARG73 | pykF::FRT-Km-FRT-PT7lac-aroAC |
| M-ARG74 | pykF::FRT-Km-FRT-PT7lac-aroLC |
| M-ARG75 | ascF::FRT-Km-FRT-PT7lac-aroED |
| M-ARG76 | ascF::FRT-Km-FRT-PT7lac-aroEB |
| M-ARG77 | ascF::FRT-Km-FRT-PT7lac-aroDB |
| M-ARG79 | pykF::FRT-Km-FRT-PT7lac-aroC |
| M-ARG80 | pykF::FRT-Km-FRT-PT7lac-aroD |
| M-ARG81 | pykF::FRT-Km-FRT-PT7lac-aroE |
| M-ARG82 | pykF::FRT-Km-FRT-PT7lac-aroL |
| M-ARG83 | pykF::FRT-Km-FRT-PT7lac-aroB |
| M-ARG84 | pykF::FRT-Km-FRT |
| M-ARG85 | ascF::FRT-Km-FRT |
| M-ARG99 | ascF::FRT-Km-FRT-PT7lac-aroB |
| M-ARG103 | pykA::FRT-Km-FRT-PT7lac-tyrB |
| M-ARG104 | pykA::FRT-Km-FRT |
| M-ARG105 | tyrB::FRT-Km-FRT |
| M-ARG109 | ldhA-feaB::FRT-Km-FRT-PT7lac-tyrAfbr |
| M-ARG110 | mtlA::FRT-Km-FRT-PT7lac-ppd(ab) |
| M-ARG111 | pykA::FRT-Km-FRT-PT7lac-yahK |
| Phenylalanine-producing strains [derived from MG1655(DE3)] | |
| M-PAR-13 | tyrR::PT7lac-aroGfbr |
| M-PAR-14 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr |
| M-PAR-20 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA |
| M-PAR-22 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr pflBA::PT7lac-tktA |
| M-PAR-24 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac-tktA |
| M-PAR-66 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA |
| M-PAR-88 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroA |
| M-PAR-104 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr ascF::PT7lac-aroEDB |
| M-PAR-106 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac-tktA ascF::PT7lac-aroEDB |
| M-PAR-108 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA ascF::PT7lac-aroEDB |
| M-PAR-110 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr pykF::PT7lac-aroALC |
| M-PAR-114 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC |
| M-PAR-116 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr pykF::PT7lac-aroALC ascF::PT7lac-aroEDB |
| M-PAR-118 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB |
| M-PAR-120 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB |
| M-PAR-140 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroAL ascF::PT7lac-aroEDB |
| M-PAR-141 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroAC ascF::PT7lac-aroEDB |
| M-PAR-142 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroLC ascF::PT7lac-aroEDB |
| M-PAR-143 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroED |
| M-PAR-144 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEB |
| M-PAR-145 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroDB |
| M-PAR-147 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroC |
| M-PAR-148 | tyrR::PT7lac-aroGfbr ldhA::PT7lac pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroD |
| M-PAR-149 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroE |
| M-PAR-150 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroL |
| M-PAR-151 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroB |
| M-PAR-152 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA ΔpykF |
| M-PAR-153 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA ΔascF |
| M-PAR-154 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroB |
| M-PAR-155 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroAC ascF::PT7lac-aroEB |
| M-PAR-156 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroAC ascF::PT7lac-aroDB |
| M-PAR-157 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroAC ascF::PT7lac-aroB |
| M-PAR-244 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB pykA::PT7lac-tyrB |
| M-PAR-245 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::P T7lac-aroEDB ΔpykA |
| M-PAR-246 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB ΔtyrB |
| M-PAR-252 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB ΔpflDC |
| Tyrosine-producing strain [derived from MG1655(DE3)] | |
| M-PAR-121 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-tyrAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB |
| Aromatic compound-producing strains [derived from MG1655(DE3)] | |
| M-PAR-239 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-pheAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB acs::PT7lac-ldhA(re) |
| M-PAR-240 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-tyrAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB acs::PT7lac-ldhA(re) |
| M-PAR-241 | tyrR::PT7lac-aroGfbr ldhA::PT7lac-tyrAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB acs::PT7lac-tdc(lb) |
| M-PAR-242 | tyrR::PT7lac-aroGfbr ldhA-feaB::PT7lac-tyrAfbr adhE::PT7lac-ppsA pflDC::PT7lac(-8TC)-tktA pykF::PT7lac-aroALC ascF::PT7lac-aroEDB pykA::PT7lac-yahK mtlA::PT7lac-ppd(ab) ΔpheA |
a
(ab) indicates an Azospirillum brasilense gene, (lb) indicates a Lactobacillus brevis gene, and (re) indicates a Cupriavidus necator/Ralstonia eutropha gene.