TABLE 3.

Comparison of recent Phe- and Tyr-producing E. coli strains

Strain	Target	Glucoseb (g/liter)	Timec (h)	Titer (g/liter)	Yield (mol/mol glucose)	Specific productivity (μmol/g DCW/h)	Culture condition, etc.d	Source or reference
Plasmid-based strains
PB12-ev2	Phe	1	10	<0.5	0.36	248	M, Res	8
AJ12714/pHYGG	Phe	40	84	6.4	0.17	ND	M	49
WSH-Z06	Phe	35	48	6.72	0.25	ND	N, Tyr, Cit	10
VH33A	Phe	5	12	ND	0.25	ND	M, Res	17
PAPV	Phe	20	40	6.23	0.34	ND	N, Org	12
T2	Tyr	5	20	0.621	0.12	ND	M	11
T2-YK	Tyr	5	16	0.7	0.14	ND	M	21
VH33ΔtyrR_TIR	Tyr	10	ND	0.15	0.36	359	M	57
F	Tyr	5	48	2.169	0.43	304	M	40
rpoA14R	Tyr	5	48	0.902	0.18	ND	M, Phe	51
CTF06	Tyr	20	96	1.62	0.17	ND	M, Phe, Trp Pyr	35
Plasmid-free strains
NST74	Phe	2	42	0.044	0.02	ND	M	14
AJ12714	Phe	40	84	3.7	0.10	ND	M	49
YHP05	Phe	20	48	0.52	0.03	ND	M, Tyr, Trp	27
WF123456	Phe	10	28	0.8	0.09	ND	M, Tyr, Trp	18
M-PAR-120a	Phe	10 (10)	28 (24)	2.26 (3.13)	0.25 (0.34)	544 (400)	M (N)	This study
DPD4195	Tyr	2	42	0.147	0.07	ND	M, Phe, Trp	14
DPD4193	Tyr	2	42	0.18	0.09	ND	M, Phe	14
SCK5	Tyr	40	60	3.0	0.10	193	N, Cit	50
BAK11	Tyr	5	24	0.402	0.08	ND	N, Phe	58
M-PAR-121	Tyr	10	28	2.2	0.22	ND	M	This study

^aValues in parentheses were obtained from the strain cultivated in M9M2-0.5Y medium. ND, not determined.

^bInitial glucose concentration.

^cCultivation time after which the yields and titers were determined.

^dAbbreviations: M, minimum medium; N, nutrient medium (minimum medium + dried yeast extract); Cit, addition of citrate; Pyr, addition of pyruvate; Org, addition of several organic compounds; Phe, Phe auxotroph; Tyr, Tyr auxotroph; Trp, Trp auxotroph; Res, resting cell reaction.