TABLE 1.
Biochemical features of wild-type PM1L, evolved OB-1, and resurrected nodes
| Feature | PM1La | OB-1 | LacAnc98 | LacAnc100 |
|---|---|---|---|---|
| MW (kDa)b | 56 | 60 | 65 | 65 |
| pIb | 5.4 | 5.1 | 5.1 | 4.8 |
| Glycosylation (%) | 6 | 12 | 15 | 15 |
| t1/2 at 50°C and pH 3.0 (min) | ND | 9 | 52 | 65 |
| t1/2 at 70°C and pH 6.0 (min) | ND | 25 | 4 | 5 |
| Optimum pH for ABTS | 2–3 | 2–3 | 2–3 | 2–3 |
| Optimum pH for DMP | 4 | 4 | 4 | 5 |
| Optimum pH for K4Mo(CN)8 | ND | 2 | 2 | 2 |
| Optimum pH for guaiacol | ND | 4 | 5 | 5 |
a
PM1L from the original basidiomycete PM1. Data were extracted from reference 40. ND, not determined.
b
The predictions of pI and molecular weight (MW) were calculated using the ExPASy Compute pI/molecular weight (MW) tool, available at https://web.expasy.org/compute_pi/.