TABLE 3.

List of primers used for amplification of cDNA targets for RT-PCR and arsenic promoters for biosensor constructions

Primer or promoter	Sequence	Target	Bacterium
Primers
ARN16S_AMB1_F1077	GGGCACTCTGAAGAAACTGC	16S RNA gene	AMB-1
ARN16S_AMB1_R1185	GTCACCACCATTGTAGCACG	16S RNA gene	AMB-1
mamK_AMB1_F390	GGCTCAGGAGGTGGTTCATA	mamK	AMB-1
mamK_AMB1_R588	GAGCCGCTCATCCACATAGT	mamK	AMB-1
arsR_AMB1_F2	TGCTTGAGACATTCGAAACCG	arsR1	AMB-1
arsR_AMB1_R112	GCACCGTGGTGATCTGGC	arsR1	AMB-1
arsC_AMB1_F23	CCTGTTTTTGTGCACCGGC	arsC1	AMB-1
arsC_AMB1_R154	GCACCGTCGGGTCGATATG	arsC1	AMB-1
ARNr16S_MSR1_F1048	GTACCGTCATCATCGTCCCC	16S RNA gene	MSR-1
ARNr16S_MSR1_R1200	CACACTGGGACTGAGACACG	16S RNA gene	MSR-1
mamK_MSR1_F773	TCGAGATTTTGTTGCGGTCC	mamK	MSR-1
mamK_MSR1_R899	ACGGGCGCAGTTTATCTTTC	mamK	MSR-1
arsR_MSR1_2119_F272	CGACGACGATCCCACTATCG	arsR1	MSR-1
arsR_MSR1_2119_R384	CTGAGACGTCAAGTCCAGGG	arsR1	MSR-1
arsC_MSR1_F43	ATTCCGCCCGTTCGATCATG	arsC1	MSR-1
arsC_MSR1_R176	TTGGTCTTCTTCAGCAGGGC	arsC1	MSR-1
arsR_MSR1_3975_F117	GAGGAATTGGCGGTCACTCC	arsR′	MSR-1
arsR_MSR1_3975_R236	TGATCGTAACGGGCCGAATAG	arsR′	MSR-1
arsM_MSR1_F595	CGACCCGTACCGAATTGTTC	arsM	MSR-1
arsM_MSR1_R719	TCGCGACTTTCTGACTTGGG	arsM	MSR-1
Promoters
pArs_AMB1_F	TATGGTCTCTGAGGTGCCATGCCGACCAAGCCGG	pArs-arsR1	AMB-1
pArs_AMB1_R	CATAGGTCTCCTCATGGTGGTCCCCCACAGCAG	pArs-arsR1	AMB-1
pArsM_MSR1_F	TATGGTCTCTGAGGGCCGGTGTCGATGATGTTG	pArs-arsR’	MSR-1
pArsM_MSR1_R	CATAGGTCTCCTCATGGCATCCTCCTGTTCAGTCG	pArs-arsR’	MSR-1