Cross-Section Schematic of an ENaCt Experiment (Top) and ENaCt Experiment with 200 nL Mineral Oil and 50 mg/mL ROY in DMSO (Bottom).
(1) Viscous inert oil dispensed onto a well of a 96-well glass plate, (2) solution of analyte in organic solvent injected into an oil droplet, (3) evaporative solvent loss to supersaturation, (4) nucleation, and (5) crystal growth. (A) solution of solvated analyte under oil, (B) evaporative solvent loss to supersaturation, (C) onset of crystal growth, and (D) complete crystallization.