Table 3.
Reagents of a Single 10 μl qPCR Reaction
| Reagents | Volume |
|---|---|
| IQ SYBR Green super mix | 5 μl |
| MQ | 2.8 μl |
| Forward + Reverse primer mix (10 μM each) | 0.2 μl |
| Template | 2 μl |
| Optional: Precision blue | 0.014 μl (1/70 μl) |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.