Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Example of a 2-step qPCR Program with Melting Curve
| Step | Temperature | Time |
|---|---|---|
| 1 | 95oC | 3:00 |
| 2 | 95oC | 0:15 |
| 3 | 60oC | 0:30 + measure fluorescence |
| 4 | Go to step 2, 39x more times | |
| 5 | 95oC | 1:00 |
| 6 | Melting curve: 70oC to 95oC, increment 0.5oC + measure fluorescence | |
