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FA lysis buffer
| Reagent | Final Concentration | Stock Concentration | Add to 500 ml |
|---|---|---|---|
| HEPES-KOH pH 7.5 | 50 mM | 1 M | 25 ml |
| NaCl | 150 mM | 5 M | 15 ml |
| EDTA pH 8.0 | 1 mM | 0.5 M | 1 ml |
| Triton X-100 | 1 % (v/v) | 10% (v/v) | 50 ml |
| Na-deoxycholate | 0.1% (w/v) | 10% (w/v) | 5 ml |
| SDS | 0.1% (w/v) | 10% (w/v) | 5 ml |
| MilliQ | 399 ml |
We filter sterilize the FA lysis buffer. Store at 4oC. Right before use, add 30 μl Aprotinin, 1 μl Pepstatin, 1 μl Leupeptin, and 10 μl PMSF per 1 ml of buffer (see Other Solutions below).
