4.5M Tris pH 8.0
| Reagent | Stock Concentration | Add to 1 L |
|---|---|---|
| Tris | 545.13 g | |
| HCl | 12.1 M | ∼211.5 ml |
| MilliQ | Up to 1 L |
We filter sterilize the 4.5 M Tris buffer, although autoclaving should also be possible. Store at 19–22°C.
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