ChIP wash buffer 2 (optional)

Reagent	Final Concentration	Stock Concentration	Add to 50 ml
Tris pH 8.0	10 mM	1 M	0.5 ml
LiCl	250 mM	5 M	2.5 ml
Nonidet P-40∗	0.5% (v/v)	10% (v/v)	2.5 ml
Na-deoxycholate	0.5% (w/v)	10% (w/v)	2.5 ml
EDTA pH 8.0	1 mM	0.5 M	0.1 ml
MilliQ			41.9 ml

Store at 19–22°C. Preferably add 30 μl Aprotinin, 1 μl Pepstatin, 1 μl Leupeptin, and 10 μl PMSF per 1 ml of buffer, right before use.

^∗Nonidet P-40 is discontinued. Substitutes are available, but we did not test these.