Other solutions

Name	Reagents
TBS	150 mM NaCl, 10 mM Tris pH 7.5
Aprotinin	3–21 TIU/mL as purchased from supplier. Store at 4oC.
Pepstatin A	1 mg/ml pepstatin A in 100% methanol (1.51 mM). Store at −20oC.
Leupeptin	1 mg/ml leupeptin in MQ (2.10 mM). Store at −20oC.
PMSF	200 mM Phenylmethanesulfonyl fluoride (PMSF) in isopropanol. Heat to 37oC to dissolve and store at −20oC.
Proteinase K	10 μg/μl in TE (10 mM Tris pH 8.0, 1 mM EDTA pH 8.0)
PBS	137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.47 mM KH2PO4 1 mM CaCl, 0.5 mM MgCl, pH adjusted to 7.3 using HCl
PBS-T	PBS + 0.02% Tween-20 (v/v)
TE/SDS	10 mM Tris pH 8.0, 1 mM EDTA pH 8.0, 1% SDS (w/v)
BSA in TBS-T	Dissolved to 10 mg/ml in TBS-T (150 mM NaCl, 10 mM Tris pH 7.5, 0.05% Tween-20)
5X sample buffer (optional)	5% SDS (w/v), 200 mM Tris pH 6.8, 25% glycerol (v/v), 1.43 M β-mercaptoethanol, 0.032% bromophenol blue (w/v)