| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| V5 Tag monoclonal Antibody IP grade (mouse) | Thermo Fisher Scientific | Cat# R960-25, RRID:AB_2556564 |
| Goat Anti-Mouse IgG (H L)-HRP Conjugate antibody | Bio-Rad | Cat# 170-6516, RRID:AB_11125547 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) | US Biological | Cat#Y2025 |
| 37% formaldehyde | Sigma-Aldrich | Cat#252549 |
| UltraPure™ Tris Buffer (powder format) | Invitrogen | Cat#15504020 |
| Zymolyase 20T | MP biomedical | Cat#08320921 |
| Aprotinin | Sigma-Aldrich | Cat#A6279 |
| Pepstatin A | Sigma-Aldrich | Cat#P4265-1MG |
| Leupeptin | Sigma-Aldrich | Cat#L2884-1MG |
| PMSF | Sigma-Aldrich | Cat#P7626 |
| Proteinase K | Roche | Cat#03115852001 |
| Broad Range Protein Marker | New England Biolabs | Cat#P7708 |
| Dynabeads Protein G | Thermo Fisher Scientific | Cat#10003D |
| RNAse A/T1 | Thermo Fisher Scientific | Cat#EN0551 |
| Critical Commercial Assays | ||
| QIAquick PCR Purification Kit | Qiagen | Cat#28106 |
| TGX Stain-Free™ FastCast™ Acrylamide Kit (10%) | Bio-Rad | Cat#1610182 |
| Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate | Perkin Elmer | Cat#NEL105001EA |
| High Sensitivity DNA Kit | Agilent | Cat#5067-4626 |
| IQ SYBR Green super mix | Bio-Rad | Cat#1708886 |
| Precision Blue™ Real-Time PCR Dye | Bio-Rad | Cat#1725555 |
| Software and Algorithms | ||
| ImageJ software | Schneider et al, 2012 | https://imagej.nih.gov/ij/ |
| CFX Maestro software | Bio-Rad | Cat#12004110 |
| Other | ||
| Cell Density Meter | VWR | Cat#634-0882 |
| Heated magnetic stir plate C-MAG HS 7 | IKA | Cat#0003581200 |
| Zirconium/silica beads 0.5 mm | BioSpec Products | Cat#11079105z |
| Analog Disruptor Genie | Scientific Industries/VWR | Cat#444-0943 |
| BD Microlance Hypod. naald - 23G 1 - 0,6 x 25 m | Becton Dickinson | Cat#300800 |
| Cooled micro centrifuge for 1.5-2.0 ml tubes | N/A | N/A |
| Cooled tabletop centrifuge for 50 ml tubes | N/A | N/A |
| Vortex | N/A | N/A |
| Bioruptor pico microtubes | Diagenode | Cat#C30010016 |
| Bioruptor pico sonicator device | Diagenode | Cat#B01060010 |
| Thermoshaker (preferred) or heat block | N/A | N/A |
| Rotating wheel (one at 4oC and one at 19-22oC) | N/A | N/A |
| Bioanalyzer | Agilent | Cat#G2939B |
| DynaMag-2 magnetic stand | Thermo Fisher Scientific | Cat#12321D |
| 1.5 ml DNA LoBind Tube | Eppendorf | Cat#0030108051 |
| CoolRack XT PCR384 for 384-wel | Corning | Cat#432055 |
| Hard-Shell® Thin-Wall 384-Well Skirted PCR Plates, white | Bio-Rad | Cat#HSP3805 |
| Microseal® 'B' PCR Plate Sealing Film | Bio-Rad | Cat#msb1001 |
| CFX384 Touch Real-Time PCR Detection system |
Bio-Rad | Cat#698-2901 |