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. 2020 Jun 3;1(1):100020. doi: 10.1016/j.xpro.2020.100020
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

V5 Tag monoclonal Antibody IP grade (mouse) Thermo Fisher Scientific Cat# R960-25, RRID:AB_2556564
Goat Anti-Mouse IgG (H L)-HRP Conjugate antibody Bio-Rad Cat# 170-6516, RRID:AB_11125547

Chemicals, Peptides, and Recombinant Proteins

Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) US Biological Cat#Y2025
37% formaldehyde Sigma-Aldrich Cat#252549
UltraPure™ Tris Buffer (powder format) Invitrogen Cat#15504020
Zymolyase 20T MP biomedical Cat#08320921
Aprotinin Sigma-Aldrich Cat#A6279
Pepstatin A Sigma-Aldrich Cat#P4265-1MG
Leupeptin Sigma-Aldrich Cat#L2884-1MG
PMSF Sigma-Aldrich Cat#P7626
Proteinase K Roche Cat#03115852001
Broad Range Protein Marker New England Biolabs Cat#P7708
Dynabeads Protein G Thermo Fisher Scientific Cat#10003D
RNAse A/T1 Thermo Fisher Scientific Cat#EN0551

Critical Commercial Assays

QIAquick PCR Purification Kit Qiagen Cat#28106
TGX Stain-Free™ FastCast™ Acrylamide Kit (10%) Bio-Rad Cat#1610182
Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate Perkin Elmer Cat#NEL105001EA
High Sensitivity DNA Kit Agilent Cat#5067-4626
IQ SYBR Green super mix Bio-Rad Cat#1708886
Precision Blue™ Real-Time PCR Dye Bio-Rad Cat#1725555

Software and Algorithms

ImageJ software Schneider et al, 2012 https://imagej.nih.gov/ij/
CFX Maestro software Bio-Rad Cat#12004110

Other

Cell Density Meter VWR Cat#634-0882
Heated magnetic stir plate C-MAG HS 7 IKA Cat#0003581200
Zirconium/silica beads 0.5 mm BioSpec Products Cat#11079105z
Analog Disruptor Genie Scientific Industries/VWR Cat#444-0943
BD Microlance Hypod. naald - 23G 1 - 0,6 x 25 m Becton Dickinson Cat#300800
Cooled micro centrifuge for 1.5-2.0 ml tubes N/A N/A
Cooled tabletop centrifuge for 50 ml tubes N/A N/A
Vortex N/A N/A
Bioruptor pico microtubes Diagenode Cat#C30010016
Bioruptor pico sonicator device Diagenode Cat#B01060010
Thermoshaker (preferred) or heat block N/A N/A
Rotating wheel (one at 4oC and one at 19-22oC) N/A N/A
Bioanalyzer Agilent Cat#G2939B
DynaMag-2 magnetic stand Thermo Fisher Scientific Cat#12321D
1.5 ml DNA LoBind Tube Eppendorf Cat#0030108051
CoolRack XT PCR384 for 384-wel Corning Cat#432055
Hard-Shell® Thin-Wall 384-Well Skirted PCR Plates, white Bio-Rad Cat#HSP3805
Microseal® 'B' PCR Plate Sealing Film Bio-Rad Cat#msb1001
CFX384 Touch Real-Time PCR
Detection system
Bio-Rad Cat#698-2901