REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
V5 Tag monoclonal Antibody IP grade (mouse) | Thermo Fisher Scientific | Cat# R960-25, RRID:AB_2556564 |
Goat Anti-Mouse IgG (H L)-HRP Conjugate antibody | Bio-Rad | Cat# 170-6516, RRID:AB_11125547 |
Chemicals, Peptides, and Recombinant Proteins | ||
Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) | US Biological | Cat#Y2025 |
37% formaldehyde | Sigma-Aldrich | Cat#252549 |
UltraPure™ Tris Buffer (powder format) | Invitrogen | Cat#15504020 |
Zymolyase 20T | MP biomedical | Cat#08320921 |
Aprotinin | Sigma-Aldrich | Cat#A6279 |
Pepstatin A | Sigma-Aldrich | Cat#P4265-1MG |
Leupeptin | Sigma-Aldrich | Cat#L2884-1MG |
PMSF | Sigma-Aldrich | Cat#P7626 |
Proteinase K | Roche | Cat#03115852001 |
Broad Range Protein Marker | New England Biolabs | Cat#P7708 |
Dynabeads Protein G | Thermo Fisher Scientific | Cat#10003D |
RNAse A/T1 | Thermo Fisher Scientific | Cat#EN0551 |
Critical Commercial Assays | ||
QIAquick PCR Purification Kit | Qiagen | Cat#28106 |
TGX Stain-Free™ FastCast™ Acrylamide Kit (10%) | Bio-Rad | Cat#1610182 |
Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate | Perkin Elmer | Cat#NEL105001EA |
High Sensitivity DNA Kit | Agilent | Cat#5067-4626 |
IQ SYBR Green super mix | Bio-Rad | Cat#1708886 |
Precision Blue™ Real-Time PCR Dye | Bio-Rad | Cat#1725555 |
Software and Algorithms | ||
ImageJ software | Schneider et al, 2012 | https://imagej.nih.gov/ij/ |
CFX Maestro software | Bio-Rad | Cat#12004110 |
Other | ||
Cell Density Meter | VWR | Cat#634-0882 |
Heated magnetic stir plate C-MAG HS 7 | IKA | Cat#0003581200 |
Zirconium/silica beads 0.5 mm | BioSpec Products | Cat#11079105z |
Analog Disruptor Genie | Scientific Industries/VWR | Cat#444-0943 |
BD Microlance Hypod. naald - 23G 1 - 0,6 x 25 m | Becton Dickinson | Cat#300800 |
Cooled micro centrifuge for 1.5-2.0 ml tubes | N/A | N/A |
Cooled tabletop centrifuge for 50 ml tubes | N/A | N/A |
Vortex | N/A | N/A |
Bioruptor pico microtubes | Diagenode | Cat#C30010016 |
Bioruptor pico sonicator device | Diagenode | Cat#B01060010 |
Thermoshaker (preferred) or heat block | N/A | N/A |
Rotating wheel (one at 4oC and one at 19-22oC) | N/A | N/A |
Bioanalyzer | Agilent | Cat#G2939B |
DynaMag-2 magnetic stand | Thermo Fisher Scientific | Cat#12321D |
1.5 ml DNA LoBind Tube | Eppendorf | Cat#0030108051 |
CoolRack XT PCR384 for 384-wel | Corning | Cat#432055 |
Hard-Shell® Thin-Wall 384-Well Skirted PCR Plates, white | Bio-Rad | Cat#HSP3805 |
Microseal® 'B' PCR Plate Sealing Film | Bio-Rad | Cat#msb1001 |
CFX384 Touch Real-Time PCR Detection system |
Bio-Rad | Cat#698-2901 |