REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
α-factor	Peptide Chemistry Science Technology Platform, The Francis Crick institute Sequence: NH2-WHWLQLKPGQPMY-COOH	N/A
cOmplete EDTA-Free Protease Inhibitor Cocktail	Sigma-Aldrich	Cat# 04693132001
Protein Assay Dye	Bio-Rad	Cat# 5000006
Trichloroacetic acid	Sigma-Aldrich	Cat# T0699
Urea	Fisher Scientific	Cat# BP169
HEPES pH 8.2	Sigma-Aldrich	Cat# H3375
EDTA	Sigma-Aldrich	Cat# E9884
Sodium fluoride	Sigma-Aldrich	Cat# S1504
Sodium vanadate	New England BioLabs	Cat# P0758
PMSF	Sigma-Aldrich	Cat# P7626
Pierce Trypsin Protease	ThermoFisher	Cat# 90058
Dithiothreitol	Sigma-Aldrich	Cat# D5545
Iodoacetamide	Sigma-Aldrich	Cat# A3221
HEPES pH 8.5	Fisher Scientific	Cat# 15433969
Trifluoroacetic acid	Fisher Scientific	Cat# A116
Acetonitrile	Fisher Scientific	Cat# A955
Acetic acid	Fisher Scientific	Cat# A11350
50 % Hydroxylamine	ThermoFisher	Cat# 90115
Formic acid	Fisher Scientific	Cat# A117
DMSO	Honeywell	Cat# 41640
Water	Fisher Scientific	Cat# W6
Critical Commercial Assays
TMT10plex Isobaric Label Reagent Set 1 x 0.8 mg	ThermoFisher	Cat# 90110
Pierce TiO2 Phosphopeptide Enrichment Spin Kits	ThermoFisher	Cat# 88303
High-Select Fe-NTA Phosphopeptide Enrichment Kit	ThermoFisher	Cat# A32992
Experimental Models: Organisms/Strains
Saccharomyces cerevisiae strains W303	https://doi.org/10.17632/tspgywx7g3.1	N/A
Software and Algorithms
Perseus v1.4.0.2	Perseus	https://maxquant.net/perseus/
MaxQuant v1.5.0.13	MaxQuant	https://www.maxquant.org
Other
Fast-Prep cell breaker	N/A	N/A
Refrigerated centrifuge capable of achieving 18,000 ×g	N/A	N/A
Benchtop centrifuge	N/A	N/A
Spectrophotometer	Ultrospec 7000	Cat# 29003605
Heat block (37°C and 56°C)	N/A	N/A
Thermomixer	N/A	N/A
SpeedVac vacuum concentrator	N/A	N/A
Ultrasonic water bath	Ultrawave	Cat# U300
Sep-Pak C18 Vac Cartridges	Waters	Cat# WAT054955
Vacuum manifold	Phenomenex	Cat# AH0-6024
Empore C18 membrane	Supelco	Cat# 66883-U
UltiMate 3000 HPLC System	Thermo Scientific	N/A
EASY-Spray C18 column 75 μm × 50 cm	ThermoFisher	Cat# ES803
Orbitrap Fusion Lumos Tribrid Mass Spectrometer	Thermo Scientific	N/A
Total recovery glass autosampler vials	Waters	Cat# 186005663CV