Table 1.

Reagents of a Single 20 μL TaqMan Real-Time PCR Reaction

Reagents	Final Concentration	Volume (μL)
AAV-ITR Forward (10 μM)	0.3 μM	0.6
AAV-ITR Reverse (10 μM)	0.3 μM	0.6
AAV-ITR TaqMan Probe (10 μM)	0.25 μM	0.5
2X TaqMan Universal PCR MasterMix	1x	10
AAV or plasmid serial dilutions	n/a	5
Dnase/Rnase-free water	n/a	3.3
Total	n/a	20