Table 2.
PCR Condition for TaqMan Real-Time PCR
Step | Temperature | Time |
---|---|---|
1 | 50°C | 2 minutes |
2 | 95°C | 10 minutes |
3 | 95°C | 15 seconds |
4 | 60°C | 1 minutes |
5 | Go to step 3 for 39 more times |
PCR Condition for TaqMan Real-Time PCR
Step | Temperature | Time |
---|---|---|
1 | 50°C | 2 minutes |
2 | 95°C | 10 minutes |
3 | 95°C | 15 seconds |
4 | 60°C | 1 minutes |
5 | Go to step 3 for 39 more times |