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Red Blood Cell Lysis Buffer
| Reagent | Final Concentration (mM) | Volume |
|---|---|---|
| NHCl4 (powder) | 155 | 8.3 g |
| KHCO3 (powder) | 10 | 1 g |
| EDTA (powder) | 0.1 | 0.037 g |
| ddH2O | n/a | 1 L |
| Total | n/a | 1 L |
Mix to dissolve and adjust pH to 7.5 with HCl. Autoclave and store this solution at 4°C for 6 months.
