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Amaxa Electroporation Buffer
| Reagent | Final Concentration (mM) | Volume (mL) |
|---|---|---|
| NaH2PO4 (1 M) | 25.2 | 0.252 |
| Na2HPO4 (1 M) | 64.8 | 0.648 |
| KCl (1 M) | 5 | 0.05 |
| MgCl2 (1 M) | 10 | 0.1 |
| HEPES (200 mM) | 20 | 1 |
| C4H4Na2O4 (240 mM) | 24 | 1 |
| ddH2O | n/a | 6.96 |
| Total | n/a | 10 |
Mix well in the hood and sterile filter. Store this solution at 4°C for 3 months.
