Dialysis/Concentration Buffer
Reagent | Final Concentration | Volume (mL) |
---|---|---|
NaCl (5 M) | 50 mM | 5 |
10% Pluronic F68 | 0.0001% | 0.05 |
PBS (pH 7.2) | n/a | 500 |
Total | n/a | 500 |
Mix well in the hood and sterile filter. Store this solution at 22°C–24°C for 6 months.