PBS-MgK Buffer

Reagent	Final Concentration (mM)	Volume (mL)
MgCl2 (1 M)	1	0.5
KCl (1 M)	2.5	1.25
PBS (pH 7.2)	n/a	500
Total	n/a	500

Mix well in the hood and sterile filter. Store this solution at 22°C–24°C for 6 months.