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. 2019 Dec 1;17(4):e2429. doi: 10.30498/IJB.2019.92707

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Copyright: © 2019 The Author(s); Published by Iranian Journal of Biotechnology

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE and Western blotting assays of L. sericata collagenase (MMP-1). (A) SDS-PAGE analysis of recombinant collagenase (MMP-1) in the cell lysate. Lane 1, 48 h after transfection ( The arrows show the target protein); lane 2, 72 h after transfection; lane 3, bacmid control (without target gene); lane 4, normal cells (B) SDS-PAGE analysis of the purified recombinant collagenase (MMP-1) Lane 1, elution 1; lane 2, NTC. (C) Western blotting of the purified recombinant collagenase (MMP-1). Lane 1, elution 1; lane 2, NTC (cell lysate of the transfected cell with bacmid-without target gene)