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Comparison of traditional and fast immunoassay method analysis times and antibody consumption for dot blotting membranes.
| Traditional Immunoassay | Flow Immunoassay | |
|---|---|---|
| Time Per Step | ||
| Blocking | 1 h | 15 min |
| Primary Antibody | 17 h | 25 min |
| Washing | 20 min | 2.5 min |
| Secondary Antibody | 1 h | 15 min |
| Washing | 20 min | 2.5 min |
| Total Time | 20 h | 1 h |
| Antibody Consumption | ||
| Primary Antibody | 5 μg | 175 ng |
| Secondary Antibody | 2 μg | 75 ng |
These values are based on a membrane trace that is 7-cm in length.
