Table 2.

Comparison of traditional, modified microscale, and fast immunoassay methods analysis times and antibody consumption for microchip western blotting membranes.

	Traditional Immunoassay	Modified Flow Immunoassay	X-link Flow Immunoassay
Time Per Step
Cross-Linking	-	-	10 s
Rinsing	-	-	110 s
Blocking	1 h	15 min	15 min
Primary Antibody	17 h	25 min	25 min
Washing	20 min	10 min	2.5 min
Secondary Antibody	1 h	1 h	15 min
Washing	20 min	10 min	2.5 min
Total Time	20 h	2 h	1 h 2 min
Antibody Consumption
Primary Antibody	5 μg	175 ng	175 ng
Secondary Antibody	2 μg	10 μg	75 ng

These values are based on a membrane trace that is 7-cm in length.