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. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Anal Methods. 2019 May 17;11(22):2862–2867. doi: 10.1039/c9ay00584f

Figure 1.

Figure 1.

Optical methods for detecting hybridization. A. Unlabeled DNA is measured with SYTO 82 intercalating dye. B, C, D. Quenching with Black Hole Quencher offset from the fluorophore by 0, 5, and 10 bases, respectively. E. Guanine quenching.