Figure 5.

Infection of A. polyphaga with L. pneumophila. Ap cells were infected with Lp at either a low MOI of 25 (white bar/symbols) or high MOI of 100 (gray bars/symbols). (A) The percent lysis of amoeba cells during the course of Lp infection was calculated as described above. Bacterial density data are presented as log₁₀ CFU mL⁻¹ and derived from cultures incubated at (B) 25 °C and (C) 30 °C. The dotted and solid lines show control (con, bacteria only) and bacteria cultured with amoeba (+Ap) data, respectively. Data were generated from two independent experiments with three replicates each (n = 6). Error bars shown represent the standard error mean. ‡, P < 0.001 Lp only versus Lp + Ap