Table 1. Genotyping primers and conditions.

Allele	Genotyping Primers	Annealing Temperature	Amplicon Size
Chd3-flox	Forward (F): 5’-GGGTGGAGGTGGAAAGTGTA-3’	55°C	Wildtype: 152 bp
	Reverse (R1): 5’-AGAGGACAGGTCACAGGACAA-3’		Floxed: 213 bp
Chd3Δ	Forward (F): 5’-GGGTGGAGGTGGAAAGTGTA-3’	59°C	~230 bp
	Reverse (R2): 5’-GGCACAGCTTCTCTGAGACC-3’
Chd4-flox	Forward: 5’-TCCAGAAGAAGACGGCAGAT-3’	55°C	Wildtype: 278 bp
	Reverse: 5’- CTGGTCATAGGGCAGGTCTC-3’		Floxed: 400 bp
Chd4Δ	Forward: 5’-TCCAGAAGAAGACGGCAGAT-3’	59°C	~350 bp
	Reverse: 5’-CAGCGTGTCAGAGTTTGCAT-3’
Tie2-Cre	Forward: 5’-GGGAAGTCGCAAAGTTGTGAGTTG-3’	60°C	533 bp
	Reverse: 5’-TCCATGAGTGAACGAACCTGGTCG-3’
Sox2-Cre	Forward: 5’-TGCAACGAGTGATGAGGTTC-3’	58°C	528 bp
	Reverse: 5’-ATTCTCCCACCGTCAGTACG-3’
FLPe	Forward: 5’-AGTAGTGATCAGGTATTGCTGTTATCTG-3’	60°C	100 bp
	Reverse: 5’-GACTAATGTTGTGGGAAATTGGAG-3’