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. 2020 Jul 13;15(7):e0236038. doi: 10.1371/journal.pone.0236038

Fig 4. Over-expression of miR-223 in LPS-activated macrophages reduces glycolysis activity and improves mitochondrial function as determined by extracellular flux analysis.

Fig 4

The extracellular flux analysis pattern of macrophages over-expressed by miR-223 or control mimic (Ctl-mimic) as evaluated by extracellular acidification rate of glucose stress test for glycolysis pathway analysis and oxygen consumption rate of mitochondrial stress test for mitochondrial pathway analysis after the stimulation by LPS versus control media (A, B) or IL-4 versus control media (C, D) with the comparison in column bar graph of glucose stress test parameters (glycolysis, respiratory capacity and glycolysis reserve) (E-G) and mitochondrial stress test parameters (basal respiration, respiratory capacity and respiratory reserve) (H-J) are demonstrated. In addition, the mitochondrial content as analyzed by the copy number of mitochondria (mtDNA) (K) and the ATP production (L) of macrophages over-expressed with Ctl-mimic or miR-223 after the stimulation with LPS, IL-4 or control media are demonstrated. Of note, the extracellular flux analysis pattern of miR-223-media is not showed due to the similarity to Ctl-mimic-media.