Fig 3. DoLA neuron number assessment of psen2 activity.

42 embryos at 24 hpf from a pair-mating of a psen2^S4Ter/+ female and a psen2^S4Ter/+ male were subjected to in situ hybridization to detect DoLA neurons that were then counted. Subsequent genotyping of individual embryos revealed 21 heterozygous mutants, 16 homozygous mutants and 5 wild type embryos. Data is shown as the mean ± SD. Values of p were determined by a one-way ANOVA followed by Dunnett’s T3 multiple comparisons test. Raw data is given in S3 Table in S1 File.