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. 2020 Jul 13;15(7):e0235784. doi: 10.1371/journal.pone.0235784

Fig 2. Bioactivity of conditioned media in a MSC migration assay.

Fig 2

Conditioned media was generated from culture media containing OFM (OFM), RAW murine macrophage cells (Mϕ), and OFM co-cultured with macrophages (OFM+Mϕ). A transwell migration assay was conducted using ovAD-MSCs, with media alone (‘Control’) and FGF2 (50 ng/mL), included as the positive and negative controls respectively. Migrated ovAD-MSCs were imaged after 6 h. Representative photomicrographs of test groups are included in panels A through E (A. Media control; B. FGF2 (50 ng/mL); C. OFM; D. Mϕ; E. OFM+Mϕ). Cell migration was quantified and results are expressed as the average cell migration normalized to the media control (‘Normalized Cell Migration’) (F). Error bars represent standard deviation from three independent experiments. Statistical significance was determined via t-test, where; ‘*’p ≤ 0.05 ‘**’p ≤ 0.01 ‘***’p ≤ 0.001; ‘****’p ≤ 0.000.