Table 1.
Target and measured water concentrations of fadrozole (FAD; µg/L) and 17β-trenbolone (TRB; ng/L) in a 4-d mixture study with fathead minnows
| Test Day | |||||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | Mean | |
| Target Treatment (FAD, TRB) | FAD, TRB | FAD, TRB | FAD, TRB | FAD, TRB | FAD, TRB | FAD, TRB | FAD, TRB |
| 0, 0 | 0, 0 (0, 0)a | 0, 0 (0, 0) | 0, 0 (0, 0) | 0, 0 (0, 0) | 0, 0 (0, 0) | 0, 0 (0, 0) | 0, 0 (0, 0) |
| 0.5, 0 | 0.51, 0 (0.02, NA) | 0.51, 0 (0.01, 0) | 0.48, 0 (0, 0) | 0.53, 0 (0, 0) | 0.60, 0 (0.03, 0) | 0.54, 0 (0.02, 0) | 0.53, 0 (0.04, 0) |
| 5, 0 | 5.01, 0 (0.28, NA) | 5.68, 0 (0.46, 0) | 5.32, 0 (0.38, 0) | 5.71, 0 (0.45, 0) | 5.81, 0 (0.44, 0) | 4.98, 0 (0.34, 0) | 5.43, 0 (0.33, 0) |
| 0, 5 | 0, 4.24 (0, NA) | 0, 3.31 (0, 0.64) | 0, 3.90 (0, 0.35) | 0, 3.51 (0, 0.28) | 0, 3.81 (0, 0.19) | 0, 2.99 (0, 3.21b) | 0, 3.82 (0, 0.58) |
| 0, 15 | 0, 11.15 (0, NA) | 0, 12.47 (0, 0.28) | 0, 11.84 (0, 0.44) | 0, 9.21 (0, 0.10) | 0, 9.95 (0, 1.69) | 0, 13.60 (0, 0.76) | 0, 11.21 (0, 1.37) |
| 0, 50 | 0, 44.67 (0, NA) | 0, 53.93 (0, 3.37) | 0, 42.38 (0, 0.320 | 0, 35.95 (0, 1.43) | 0, 36.83 (0, 2.60) | 0, 50.89 (0, 2.09) | 0, 43.30 (0, 6.42) |
| 0.5, 5 | 0.55, 5.45 (0, NA) | 0.52, 4.43 (0.01, 0.26) | 0.50, 4.20 (0, 0.27) | 0.55, 3.84 (0.02, 0.42) | 0.61, 3.84 (0.01, 0.15) | 0.57, 5.46 (0.01, 0.34) | 0.55, 4.37 (0.03, 0.55) |
| 0.5, 15 | 0.50, 14.10 (0.04, NA) | 0.51, 10.89 (0.01, 0.08) | 0.46, 10.97 (0.01, 0.60) | 0.50, 9.46 (0.01, 0.12) | 0.56, 10.89 (0.01, 0.42) | 0.52, 13.06 (0.01, 0.14) | 0.51, 11.27 (0.03, 1.37) |
| 0.5, 50 | 0.55, 46.15 (0.02, NA) | 0.52, 45.31 (0.02, 1.58) | 0.49, 51.17 (0.02, 2.33) | 0.53, 38.18 (0.03, 1.07) | 0.59, 42.26 (0.03, 1.15) | 0.54, 50.45 (0.03, 0.22) | 0.54, 45.05 (0.03, 4.76) |
| 5, 5 | 4.91, 5.14 (0.11, NA) | 5.46, 4.11 (0.11, 0.05) | 5.27, 4.15 (0.06, 0.07) | 5.65, 3.65 (0.14, 0.08) | 5.74, 3.96 (0.14, 0.21) | 4.97, 5.57 (0.11, 0.13) | 5.33, 4.45 (0.31, 0.67) |
| 5, 15 | 4.49, 12.54 (0.23, NA) | 5.01, 11.81 (0.08, 1.08) | 4.79, 11.98 (0.08, 0.23) | 5.01, 9.95 (0.21, 0.05) | 5.11, 10.51 (0.32, 0.56) | 4.39, 13.82 (0.27, 0.17) | 4.78, 11.87 (0.31, 1.26) |
| 5, 50 | 4.84, 45.11 (0.13, NA) | 5.35, 48.30 (0.17, 6.40) | 5.28, 52.97 (0.32, 1.45) | 5.65, 37.64 (0.25, 2.02) | 5.83, 39.96 (0.24, 0.69) | 5.14, 53.65 (0.26, 1.50) | 5.35, 47.34 (0.34, 6.20) |
a
Control; detection limits = 0.05 µg/L (FAD) and 0.3 ng/L (TRB). Values indicate mean (± SD) where n=3 tanks for controls and FAD and n=1–2 tanks for TRB.
b
Low detection of TRB in one of the 3 tanks; chemical flow cessation for 24 h in that tank.
NA=Not applicable to calculating SD because only one measurement was made.