Figure 3.

Modeling of Cellular Contact Guidance

(A–D) The computational (dashed green boxes) results for myofibroblasts on parallel lines of fibronectin (2–200 μm) is compared against the corresponding experimental results (solid green boxes), in terms of the distribution of the cellular orientation (A), aspect ratio (B), area (C), and number of lines touched by single cells (D). The boxes of the boxplots represent the quartiles of the distributions, with the whiskers indicating the outliers in the experiments and the 5^th and 95^th percentiles of the distributions.

(E–H) Probability density functions (PDFs) of the energies of myofibroblasts associated with the total Gibbs free energy (E), elasticity (F), cytoskeletal (G), and adhesion Gibbs free energy of the cell (H) are also shown. Each color represents a specific pattern of parallel lines (w × s μm). The energies are normalized by the energy of the free-standing cell. The data reported are results from three independent samples, and at least 60 cells were considered per condition.