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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2020 May 19;19(7):1562–1571. doi: 10.1158/1535-7163.MCT-19-1036

Figure 3. Effects of PTEN knockout on the response to cetuximab in cell proliferation and orosphere formation assays.

Figure 3.

(A) Parental and isogenic CAL27 and HN12 cells seeded in 96-well plates (2000 per well) were treated with the indicated concentrations of erlotinib for 72h. Cell viabilities were normalized with that of the corresponding vehicle control (0.1% DMSO)-treated cells. (B) Parental and isogenic HN12 cells were seeded in 96-well ultra-low attachment culture dishes at 100 cells per well (n = 10), and treated with vehicle control (0.9% NaCl) or cetuximab (10 μg/ml). 10 days after treatment, the size of spheres in each well were determined. (C) Representative spheres obtained from parental and PTEN knockout cells treated with vehicle control or cetuximab (10 μg/ml).