Table 1.

Structural Refinement Statistics of Unbound and Bound WT Tau Duplexes

	unbound RNA	4-bound RNA	5-bound RNA	9-bound RNA
no. of restraints
all distance restraints, including hydrogen bonds	191	182	258	173
all NOE restraints	150	141	221	146
intraresidue	69	68	121	75
sequential residues	79	62	79	57
long range RNA (excluding 4–, 5–, or 9–RNA)	2	2	2	2
intramolecular 4, 5, or 9	N/A	2	6	1
intermolecular 4–, 5–, or 9–RNA	N/A	7	13	11
hydrogen bonds	41	41	37	27
dihedral	0	0	0	105
RMSD of experimental restraints
distance (Å)	3.8 × 10−3	2.8 × 10−3	4.2 × 10−3	2.1 × 10−3
dihedral (deg)	N/A	N/A	N/A	2.1 × 10−3
RMSD of structures for heavy atoms (Å)
all residues, excluding 4, 5, or 9	1.79 ± 0.31	1.92 ± 0.49	1.11 ± 0.25	1.37 ± 0.36
all residues and 4, 5, or 9	N/A	1.91 ± 0.47	1.12 ± 0.25	1.41 ± 0.34
outer helices (residues 1–3, 9–14, and 19–21)	2.01 ± 0.40	1.92 ± 0.61	1.02 ± 0.31	1.40 ± 0.47
4, 5, or 9	N/A	1.06 ± 0.38	0.50 ± 0.33	1.08 ± 0.60
Bulge loop and inner base pairs, including 4, 5, or 9 for bound structure (residues 4–8 and 15–18)	0.84 ± 0.19	1.53 ± 0.37	0.85 ± 0.17	1.14 ± 0.34
Bulge loop and closing pairs, including 4, 5, or 9 for bound structure (residues 5–7 and 16–17)	0.63 ± 0.19	1.43 ± 0.39	0.97 ± 0.22	1.23 ± 0.40