KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Fasciclin 3 (7G10) | Developmental studies hybridoma bank (DSHB) | RRID: AB_528238 |
| Mouse monoclonal anti-alfa-Spectrin (3A9) | DSHB | RRID: AB_528473 |
| Mouse monoclonal anti-Eyes absent (10H6) | DSHB | RRID: AB_528232 |
| Rat monoclonal anti-Vasa | DSHB | RRID: AB_760351 |
| Mouse monoclonal anti-phospho-Histone H3 (Ser10) (6G3) | Cell Signaling Technology | Cat#9706; RRID: AB_331748 |
| Chicken polyclonal anti-GFP | Aves Labs | Cat#GFP-1020; RRID: AB_10000240 |
| Rabbit monoclonal anti-P-4E-BP1 (T37/46) (236B4) | Cell Signaling Technology | Cat#2855; RRID: AB_560835 |
| Mouse monoclonal anti-dEGF Receptor (clone C-273) | Sigma-Aldrich | Cat#E2906; RRID: AB_609900 |
| Rabbit polyclonal anti-Zfh1 | Laboratory of Ruth Lehmann (NYU) | N/A |
| Guinea pig polyclonal anti-Traffic Jam | Li et al., 2003 | RRID: AB_2568583 |
| Rabbit monoclonal anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP | Cell Signaling Technology | Cat#4370; RRID: AB_2315112 |
| Rabbit polyclonal anti-Vasa (d-260) | Santa Cruz Biotechnology | Cat#sc-30210; RRID: AB_793874 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rapamycin | TSZCHEM | Cat#R1017; CAS#53123-88-9 |
| Chloroquine diphosphate salt | Sigma-Aldrich | Cat#C6628; CAS#50-63-5 |
| L-Carnitine inner salt | BeanTown Chemical | Cat#215415; CAS#541-12-1 |
| BODIPY™ 493/503 | ThermoFisher | Cat#D3922 |
| BODIPY™ 558/568 C12 | ThermoFisher | Cat#D3835 |
| HCS LipidTox™ Red Neutral Lipid Stain | ThermoFisher | Cat#H34476 |
| Critical Commercial Assays | ||
| ApopTag® Red In Situ Apoptosis Detection Kit | Millipore/Sigma- Aldrich | Cat#S7165 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: tub-Gal80TS | Bloomington Drosophila Stock Center | BDSC:7108 |
| D. melanogaster: UAS-EGFRDN | Bloomington Drosophila Stock Center | BDSC:5364 |
| D. melanogaster: UAS-EGFRCA | Bloomington Drosophila Stock Center | BDSC:9533 |
| D. melanogaster: UAS-TorRNAi [HMS0114] | Bloomington Drosophila Stock Center | BDSC:34639 |
| D. melanogaster: UAS-Atg1RNAi [GL00047] | Bloomington Drosophila Stock Center | BDSC:35177 |
| D. melanogaster: UAS-Atg1RNAi [HMS02750] | Bloomington Drosophila Stock Center | BDSC:44034 |
| D. melanogaster: UAS-Atg8bRNAi [HMS01245] | Bloomington Drosophila Stock Center | BDSC:34900 |
| D. melanogaster: UAS-Atg7RNAi [HMS01358] | Bloomington Drosophila Stock Center | BDSC:34369 |
| D. melanogaster: UAS-RasV12 | Bloomington Drosophila Stock Center | BDSC:4847 |
| D. melanogaster: UAS-InRRNAi [JF01482] | Bloomington Drosophila Stock Center | BDSC:31037 |
| D. melanogaster: UAS-SREBP[1–452] (‘SREBPCA’) | Bloomington Drosophila Stock Center | BDSC:41018 |
| D. melanogaster: Grb1(PH)-GFP [tGPH2] | Bloomington Drosophila Stock Center | BDSC:8163 |
| D. melanogaster: TJ-Gal4 | Kyoto Stock Center | BDSC:104055 |
| D. melanogaster: UAS-Atg1 [GD7149] | Vienna Drosophila RNAi Center | v16133 |
| D. melanogaster: UAS-Atg5RNAi [KK108904] | Vienna Drosophila RNAi Center | v104461 |
| D. melanogaster: UAS-Atg7RNAi [GD11671] | Vienna Drosophila RNAi Center | v27432 |
| D. melanogaster: UAS-Atg8aRNAi [KK102155] | Vienna Drosophila RNAi Center | v109654 |
| D. melanogaster: UAS-Atg8aRNAi [GD4654] | Vienna Drosophila RNAi Center | v43097 |
| D. melanogaster: UAS-Atg9RNAi [GD2029] | Vienna Drosophila RNAi Center | v10045 |
| D. melanogaster: UAS-Atg12RNAi [GD15230] | Vienna Drosophila RNAi Center | v29781 |
| D. melanogaster: UAS-Atg13RNAi [GD12202] | Vienna Drosophila RNAi Center | v27956 |
| D. melanogaster: UAS-Atg13RNAi [KK100340] | Vienna Drosophila RNAi Center | v103381 |
| D. melanogaster: UAS-Vps34RNAi [KK107602] | Vienna Drosophila RNAi Center | v100296 |
| D. melanogaster: UAS-bmmRNAi [GD5139] | Vienna Drosophila RNAi Center | v37877 |
| D. melanogaster: UAS-coltRNAi [KK107449] | Vienna Drosophila RNAi Center | v106089 |
| D. melanogaster: UAS-whdRNAi [GD1917] | Vienna Drosophila RNAi Center | v4046 |
| D. melanogaster: pAtg8a > mCherry-Atg8a | Laboratory of T. Neufeld (U. Minnesota) | N/A |
| D. melanogaster: UAS-GFP-mCherry-Atg8a | Laboratory of T. Neufeld (U. Minnesota) | N/A |
| D. melanogaster: UAS-Atg16A (‘Atg1OE’) | Laboratory of T. Neufeld (U. Minnesota) | N/A |
| D. melanogaster: UAS-Atg6RNAi [IR 16–3] | Laboratory of T. Neufeld (U. Minnesota) | N/A |
| D. melanogaster: UAS-Fos | Laboratory of H. Jasper (The Buck Institute for Aging Research) | N/A |
| D. melanogaster: UAS-FosRNAi-strong | Laboratory of H. Jasper (The Buck Institute for Aging Research) | N/A |
| D. melanogaster: UAS-FosC-Ala | Laboratory of F. Serras (Universitat de Barcelona, Spain) | N/A |
| D. melanogaster: UAS-FosPan-Ala | Laboratory of F. Serras (Universitat de Barcelona, Spain) | N/A |
| D. melanogaster: UAS-RasV12.S35 | Laboratory of H. Steller (The Rockefeller University) | N/A |
| D. melanogaster: UAS-RasV12.G37 | Laboratory of H. Steller (The Rockefeller University) | N/A |
| D. melanogaster: UAS-FosPan-Ala | Laboratory of F. Serras (Universitat de Barcelona, Spain) | N/A |
| D. melanogaster: nos-Gal4:VP16 | Laboratory of M. van Doren (Johns Hopkins) | N/A |
| D. melanogaster: c587-Gal4 | Laboratory of T. Xie (Stowers Institute of Biomedical Research) | N/A |
| D. melanogaster: stet871 | Laboratory of M. Fuller (Stanford University) | N/A |
| D. melanogaster: Df(3L)PX62 | Laboratory of M. Fuller (Stanford University) | N/A |
| Oligonucleotides | ||
| Primer Act5C For: TTGTCTGGGCAAGAGGATCAG | This paper | N/A |
| Primer Act5C Rev: ACCACTCGCACTTGCACTTTC | This paper | N/A |
| Primer Atg6 For: CGACAATGAGTGAGGCGGAA | This paper | N/A |
| Primer Atg6 Rev: TCTCCGTAGATGGGCAAAGA | This paper | N/A |
| Software and Algorithms | ||
| Prism v6.0 | Graphpad | N/A |
| Illustrator CC 2015 | Adobe | N/A |
| Photoshop CC 2015 | Adobe | N/A |
| ImageJ v2.0.0 | Wayne Rasband, NIH | https://imagej.nih.gov/ij |
| Imaris v8.4 | Bitplane | N/A |
| CFX ManagerTM | Biorad | N/A |