Fig. 9.

Activation of autophagy by DON suppressed the antiviral innate immune response via inhibiting STING signaling phosphorylation in PEDV-infected IPEC-J2 cells. a-d Cell treated with or without AUD-S100 lysates were subjected to immunoblotting with antibodies to p-STING and STING. e Cells treated with or without AUD-S100 were assayed for PEDV viral titers. f, g Cell lysates were subjected to immunoblotting with antibodies to LC3B, p-STING/STING or β-actin (loading control) at the absence or presence of scrambled or shLC3B. h, i RT-qPCR were performed to analyze the mRNA levels of IFN-α and IFN-β at the absence or presence of scrambled or shLC3B. j, k Cell lysates were subjected to immunoblotting with antibodies to p-STING, STING or β-actin (loading control) at the absence or presence of Rapa or SB202190.The data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01.