| Genomic PCR |
MAC1 |
m11 5L |
TGACAGAGAGCTTCCTCCTGCCTCTGTA |
EGFP-F |
CCTGAAGTTCATCTGCACCA |
5.0 kb |
| Chr.21-loxP |
#21CEN < 1 > 2L |
AAATGCATCACCATTCTCCCAGTTACCC |
PGKr1 |
GGAGATGAGGAAGAGGAGAACA |
4.5 kb |
| Cre-loxP recombination |
TRANSL1 |
TGGAGGCCATAAACAAGAAGAC |
TRANSR1 |
CCCCTTGACCCAGAAATTCCA |
409 bp |
| Cre-loxP recombination |
kj neo |
CATCGCCTTCTATCGCCTTCTTGACG |
PGKr1 |
GGAGATGAGGAAGAGGAGAACA |
~600 bp |
| D21S265 |
SHGC-40F |
GGGTAAGAAGGTGCTTAATGCTC |
SHGC-40R |
TGAATATGGGTTCTGGATGTAGTG |
178 bp |
| APP |
SHGC-31514F |
CTGGGCAATAGAGCAAGACC |
SHGC-31514R |
ACCCATATTATCTATGGACAATTGA |
115 bp |
| D21S260 |
D21S260F |
AGCTGTTCATGCTTCCATCT |
D21S260R |
AGAGCCCAGAATATTGACCC |
270 bp |
| SOD1 |
SHGC-6902F |
ATTCTGTGATCTCACTCTCAGG |
SHGC-6902R |
TCGCGACTAACAATCAAAGT |
133 bp |
| D21S261 |
SHGC-3610F |
AACACCTTACCTAAAACAGCA |
SHGC-3610R |
TGGACCTTTTGATTTTTCCT |
130 bp |
| AML1 |
SHGC-30487F |
GTAACCTGGTTAACATAGGGTTTC |
SHGC-30487R |
GTAGGGGAGGCTAATGGCAT |
150 bp |
| CBR1 |
J04056F |
GATCCTCCTGAATGCCTG |
J04056R |
GTAAATGCCCTTTGGACC |
245 bp |
| SIM2 |
WI-22186F |
GGGCCTCATGGTAAGAGTCA |
WI-22186R |
GAAAAATGTCGGTGGTATCTCC |
250 bp |
| HLCS |
WI-15188F |
TTCAGTACCTCCCCAGATGC |
WI-15188R |
CTTAGTAGTGCAGACCTTTACCCC |
125 bp |
| TTC3 |
WI-19945F |
TGGACAAATATAAGGCATGTTCA |
WI-19945R |
GTCACCTTCCTCTGCCTTTG |
267 bp |
| D21S394 |
D21S394F |
GGAGCCGGTTCTTCGAAGG |
D21S394R |
CAGCGTCCGGAATTCCTGC |
71 bp |
| D21S336 |
D21S336F |
TCTGGTTCCCAGGATTGTAA |
D21S336R |
AGAGTTGCTGTAAGCATCAAAGT |
350 bp |
| D21S55 |
D21S55F |
AGGCTCCTTCACCTCTTGAC |
D21S55R |
CATCCTCTTTGCATTAGG |
159 bp |
| GIRK2 |
GIRK2F |
GTTTGTCTTCAGCTCACC |
GIRK2R |
CCCAAAATACTACACATCC |
266 bp |
| ERG |
M21535F |
AATGGCGTCAGCCTCTCC |
M21535R |
CAGTTTGCCTTACGAGTGGTAGC |
254 bp |
| ETS2 |
SHGC-11267F |
TACCATGCCAATGGTTTATAAGG |
SHGC-11267R |
ATGTGACTGGGAACATCTTGC |
177 bp |
| D21S268 |
D21S268F |
CAACAGAGTGAGACAGGCTC |
D21S268R |
TTCCAGGAACCACTACACTG |
213 bp |
| PCP4 |
WI-14954F |
GAATTCACTCATCGTAACTTCATTT |
WI-14954R |
CCTTGTAGGAAGGTATAGACAATGG |
126 bp |
| D21S266 |
D21S266F |
CACAATGTAGATGTAGCACAGTTAG |
D21S266R |
TGAGTCTGAAGAAAGGCAAATGAAG |
166 bp |
| D21S15 |
D21S15F |
GAGGATAAACCGATTCACAGCTAGGAATAC |
D21S15R |
GTGCACGTAATTAATGACCATGATATTGCT |
218 bp |
| MX1 |
WI-18875F |
TGGACTGACGACTTGAGTGC |
WI-18875R |
CTCATGTGCATCTGAGGGTG |
143 bp |
| TFF1 |
SGC35308F |
CAGGGATCTGCCTGCATC |
SGC35308R |
ATCGATCTCTTTTAATTTTTAGGCC |
183 bp |
| PWP2 |
SHGC-33273F |
GATCTTGACCGGGAAAAGGG |
SHGC-33273R |
AACAAGTGGCAAAATGCATAC |
150 bp |
| APECED |
A009B16F |
AAAATCCTCCCTTTAAGAGC |
A009B16R |
GGGTGTTAGGTACTGGCT |
118 bp |
| PFKL |
sts-X15573F |
AGGGCTTCTGAGGCCAGC |
sts-X15573R |
AGGGCACTCTGTCCTCCTGC |
239 bp |
| UBE2G2 |
WI-11417F |
TTCAACAGTCATTAGGTTCCACC |
WI-11417R |
GTGAGATCGGGAGAGGGAG |
129 bp |
| RT-PCR |
APP |
WI-18826F |
ACGTTTGTTTCTTCGTGCCT |
WI-18826R |
GCCCCGTAAAAGTGCTTACA |
136 bp |
| SOD1 |
SHGC-6902F |
ATTCTGTGATCTCACTCTCAGG |
SHGC-6902R |
TCGCGACTAACAATCAAAGT |
133 bp |
| IFNAR2 |
U29584F |
CGAAGTTTCAGTCGGTGAG |
U29584R |
GGCATTCAGGTTTTATCCC |
181 bp |
| TTC3 |
WI-19945F |
TGGACAAATATAAGGCATGTTCA |
WI-19945R |
GTCACCTTCCTCTGCCTTTG |
267 bp |
| PCP4 |
WI-14954F |
GAATTCACTCATCGTAACTTCATTT |
WI-14954R |
CCTTGTAGGAAGGTATAGACAATGG |
126 bp |
| MX1 |
WI-18875F |
TGGACTGACGACTTGAGTGC |
WI-18875R |
CTCATGTGCATCTGAGGGTG |
143 bp |
| TFF3 |
WI-7267F |
GGCTGTGATTGCTGCCAG |
WI-7267R |
GTGGAGCATGGGACCTTTAT |
124 bp |
| TFF1 |
SGC35308F |
CAGGGATCTGCCTGCATC |
SGC35308R |
ATCGATCTCTTTTAATTTTTAGGCC |
183 bp |
| GADPH |
RPC1 |
CCATCTTCCAGGAGCGAGA |
RPC2 |
TGTCATACCAGGAAATGAGC |
722 bp |
