Table 3. scRNA-seq Technologies.

Strengths and weaknesses of the ever-evolving compendium of scRNA-seq technologies and analysis packages have been evaluated reviewed extensively in Ziegenhain et al., 2017; Chen et al., 2019a; Haque et al., 2017. Here, we provide a basic overview of the strengths of the general approaches.
Technology	Plate-based (e.g. Smart-seq2, MARS-seq)	Microfluidic capture (e.g. C1, Seq-well, CEL-seq2/C1)	Droplet (e.g. 10X, Drop-Seq)
Strengths	• Highest sensitivity (number of genes detected) • fewer multiplets • full-length transcripts possible	• High sensitivity (number of genes detected) • fewer multiplets • no sorting required	• Inexpensive (per cell) • profile high numbers of cells • can identify less frequent cell types • no sorting required • Can use UMIs
Weaknesses	• Requires sorting • low throughput • high cost per cell • not strand specific	• 3' Only • limited cell numbers • (typically) not strand-specific	• 3' Only • fewer genes/UMIs • more dropout