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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Cell Immunol. 2020 May 14;353:104129. doi: 10.1016/j.cellimm.2020.104129

Fig. 6.

Fig. 6.

NEMO is dispensable for IKKα/β phosphorylation at mCADS, but required for downstream NF-κB induction. (A) Confocal IF microscopy of JPM50.6 T cells transfected with WT, E134G or G123D CARD11-FLAG constructs stained using anti-FLAG, anti-BCL-10, and anti-NEMO (IKKγ) Abs at 24 hrs post-transfection. (B) Confocal IF microscopy of NEMO-deficient JM4.5.2 T cells transfected with WT, E134G or G123D CARD11-FLAG constructs and stained using anti-FLAG, anti-MALT1, and anti-ph-IKKα/β Abs at 24 hrs post-transfection. Merge images include DAPI staining. Scale bars = 5 μm. (C) NF-κB activity was measured in cell JPM50.6 and JM4.5.2. transfectants from (A) and (B) using a dual luciferase assay. (D) Immunoblotting of lysates prepared from transfected cells for CARD11-FLAG and NEMO expression. β-actin served as a loading control. Data are representative of >3 independent experiments.