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. 2020 Jul 9;14:2695–2705. doi: 10.2147/DDDT.S255158

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© 2020 Zhang et al.

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NGF was a target gene of let-7. (A)TargetScan databases was employed to predict the target sites between let-7 microRNAs and NGF, and NGF wild-type and mutant reporter vectors were constructed. (B) Dual-luciferase reporter gene experiments were used to verify the targeting relationship between let-7 and the 3ʹUTR of NGF. (C–D) qRT-PCR and Western blot were used to detect the expression levels of NGF mRNA and protein in the sciatic nerve of rats, n = 3/group. *P < 0.05, **P < 0.01, and ***P < 0.001.

Abbreviations: Wild type, wild type of NGF 3ʹUTR; mutant, mutant of NGF 3ʹUTR; NC, negative control; let-7, let-7 mimic; NGF, nerve growth factor; Sham, sham group; Saline, saline-treated group; UTI, UTI-treated group; ns, not significant.