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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Clin Cancer Res. 2020 Jan 14;26(14):3856–3867. doi: 10.1158/1078-0432.CCR-19-1397

Fig 5. Impact of baseline endogenous levels of Bcl-2 family proteins on AMG-176-induced cell death.

Fig 5.

(A - B) CLL lymphocytes from peripheral blood of 16 patients were isolated to measure basal levels of Bcl-2 family proteins. Total protein was purified from these lymphocytes and MEC-1 cells and immunoblots were performed for the indicated Bcl-2 family proteins. (C - E) Protein levels were quantitated and normalized with GAPDH and compared with the levels in MEC-1 cells. The % Mcl-1, Bcl-2, and Bcl-xL levels were correlated with % apoptosis after a 24 hour incubation with 300 nM AMG-176. Linear regression and statistical analyses were performed using prism software, which required to put data point 0 on X-axis at 100% Y-axis value. Quantitation graphs of Bak and Bax proteins are shown in Supplemental Figure 9.