Table 1.
Results of real time RT-PCR amplification (positivity +, negativity -), infectivity test (presence or absence of CPE), genome sequencing and caffeine analysis, as obtained for WWTPs and rivers for the two sampling dates. Genes code refers to the nucleocapsid (N) gene, the Orf1ab gene and the E gene. n.a.: analysis not performed.
| Date | Sample origin | Station | Treatment | Gene positivity |
Infectivity test | Genome code | Caffeine μg/L | ||
|---|---|---|---|---|---|---|---|---|---|
| Orf1ab | N | E | |||||||
| 14/04/2020 | WWTPs | A | Raw | + | − | + | No CPE | hCoV-19/Italy/HSacco-1/2020 | 35.31 |
| A | Treated | − | − | − | No CPE | n.a. | <0.02 | ||
| B line 1 | Raw | + | − | + | n.a. | n.a. | 28.71 | ||
| B line 2 | Raw | + | + | + | No CPE | n.a. | 32.81 | ||
| C | Raw | − | − | − | n.a. | n.a. | 26.31 | ||
| C | Treated | − | − | − | n.a. | n.a. | <0.02 | ||
| Rivers | Vettabbia | − | + | + | − | No CPE | n.a. | <0.02 | |
| Lambro Meridionale | − | + | + | − | No CPE | n.a. | 0.36 | ||
| Lambro | − | + | + | − | No CPE | n.a. | 0.28 | ||
| 22/04/2020 | WWTPs | A | Raw | − | − | − | No CPE | n.a. | 24.89 |
| A | Treated | − | − | − | No CPE | n.a. | 0.03 | ||
| B line 1 | Raw | + | + | − | No CPE | n.a. | 22.72 | ||
| B line 2 | Raw | − | − | − | No CPE | n.a. | 16.24 | ||
| C | Raw | − | − | − | No CPE | n.a. | 21.61 | ||
| C | Treated | − | − | − | No CPE | n.a. | 0.06 | ||
| Rivers | Vettabbia | − | − | − | − | No CPE | n.a. | <0.02 | |
| Lambro Meridionale | − | − | − | − | No CPE | n.a. | 0.41 | ||
| Lambro | − | + | + | − | No CPE | n.a. | 0.44 | ||