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. 2020 May 1;21:428–440. doi: 10.1016/j.omtn.2020.04.014

Table 2.

The Primer Sequences of miR-203, U6, PRC1, β-actin, β-catenin, Wnt8b, c-Jun, FZD3, p16, Bcl-2, and c-Myc used for qRT-PCR

Gene Sequence
miR-203 F: 5′-TGCTCTAGAGGCGTCTAAGGCGTCCG-3′
R: 5′-CCCAAGCTTCACCTCCCAGCAGCACTTG-3′
U6 F: 5′-AAAGCAAATCATCGGACGACC-3′
R: 5′-GTACAACACATTGTTTCCTCGGA-3′
PRC1 F: 5′-CAAGCGCCGTATGGGACTTT-3′
R: 5′-GGAGGCATCCATGTAGCTCT-3′
β-actin F: 5′-AGCGAGCATCCCCCAAAGTT-3′
R: 5′-GGGCACGAAGGCTCATCATT-3′
β-catenin F: 5′-GCAACCCTGAGGAAGAAGAT-3′
R: 5′-TTAGCTCCTTCCTGATGGAG-3′
Wnt8b F: 5′-CCGACACCTTTCGCTCCATC-3′
R: 5′-CAGCCCTAGCGTTTTGTTCTC-3′
c-Jun F: 5′-AAAGAATTCATGACTGCAAAGATGGAAACG-3′
R: 5′-AAACTCGAGTCAAAATGTTTGCAACTGCTG-3′
FZD3 F: 5′-GTTCATGGGGCATATAGGTGG-3′
R: 5′-GCTGCTGTCTGTTGGTCATAA-3′
p16 F: 5′-TGTGTCCAGTCCATGCCAAC-3′
R: 5′-AGGAAATCGTAGCACTGCAAG-3′
Bcl-2 F: 5′-GTCTTCGCTGCGGAGATCAT-3′
R: 5′-CATTCCGATATACGCTGGGAC-3′
c-Myc F: 5′-ATGGCCCATTACAAAGCCG-3′
R: 5′-TTTCTGGAGTAGCAGCTCCTAA-3′
Snail F: 5′-TCCAGAGTTTACCTTCCAGCA-3′
R: 5′-CTTTCCCACTGTCCTCATCTG-3′

qRT-PCR, quantitative reverse transcription polymerase chain reaction; miR-203, microRNA-203; PRC1, polycomb repressive complex 1; FZD3, frizzled-3; Bcl-2, B cell leukemia/lymphoma 2; F, forward; R, reverse.