Table 2.
The Primer Sequences of miR-203, U6, PRC1, β-actin, β-catenin, Wnt8b, c-Jun, FZD3, p16, Bcl-2, and c-Myc used for qRT-PCR
| Gene | Sequence |
|---|---|
| miR-203 | F: 5′-TGCTCTAGAGGCGTCTAAGGCGTCCG-3′ |
| R: 5′-CCCAAGCTTCACCTCCCAGCAGCACTTG-3′ | |
| U6 | F: 5′-AAAGCAAATCATCGGACGACC-3′ |
| R: 5′-GTACAACACATTGTTTCCTCGGA-3′ | |
| PRC1 | F: 5′-CAAGCGCCGTATGGGACTTT-3′ |
| R: 5′-GGAGGCATCCATGTAGCTCT-3′ | |
| β-actin | F: 5′-AGCGAGCATCCCCCAAAGTT-3′ |
| R: 5′-GGGCACGAAGGCTCATCATT-3′ | |
| β-catenin | F: 5′-GCAACCCTGAGGAAGAAGAT-3′ |
| R: 5′-TTAGCTCCTTCCTGATGGAG-3′ | |
| Wnt8b | F: 5′-CCGACACCTTTCGCTCCATC-3′ |
| R: 5′-CAGCCCTAGCGTTTTGTTCTC-3′ | |
| c-Jun | F: 5′-AAAGAATTCATGACTGCAAAGATGGAAACG-3′ |
| R: 5′-AAACTCGAGTCAAAATGTTTGCAACTGCTG-3′ | |
| FZD3 | F: 5′-GTTCATGGGGCATATAGGTGG-3′ |
| R: 5′-GCTGCTGTCTGTTGGTCATAA-3′ | |
| p16 | F: 5′-TGTGTCCAGTCCATGCCAAC-3′ |
| R: 5′-AGGAAATCGTAGCACTGCAAG-3′ | |
| Bcl-2 | F: 5′-GTCTTCGCTGCGGAGATCAT-3′ |
| R: 5′-CATTCCGATATACGCTGGGAC-3′ | |
| c-Myc | F: 5′-ATGGCCCATTACAAAGCCG-3′ |
| R: 5′-TTTCTGGAGTAGCAGCTCCTAA-3′ | |
| Snail | F: 5′-TCCAGAGTTTACCTTCCAGCA-3′ |
| R: 5′-CTTTCCCACTGTCCTCATCTG-3′ |
qRT-PCR, quantitative reverse transcription polymerase chain reaction; miR-203, microRNA-203; PRC1, polycomb repressive complex 1; FZD3, frizzled-3; Bcl-2, B cell leukemia/lymphoma 2; F, forward; R, reverse.