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. 2020 Jul 7;11:1138. doi: 10.3389/fimmu.2020.01138

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Copyright © 2020 Omura, Sato, Park, Fujita, Khadka, Nakamura, Katsuki, Nishio, Gavins and Tsunoda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-TMEV antibody isotype responses. Correlations between CNS Ig isotype expressions and serum anti-TMEV isotype antibody titers. During the chronic phase of TMEV infection, gene expression levels of constant regions of heavy chains of IgG1 (Ighg1), IgG2b (Ighg2b), IgG2c (Ighg2c), and IgA (Igha) were correlated with their anti-TMEV antibody isotype titers, significantly (P < 0.01, n = 10). We quantified the gene expression levels and anti-TMEV antibody titers by RNA-seq and enzyme-linked immunosorbent assays (ELISA, absorbance at 490 nm), respectively.