Figure 5.

Abundance of small RNAs mapping on the T-DNA inserted in MdDAM1 transgenic lines. The number of small RNA (sRNA) mapping in sense (+ strand) or in antisense (− strand) of the T-DNA comprising the Promoter (Pro35S), the MdDAM1 coding region and the octopine synthase terminator (tOCS) are indicated. The colored lines indicate the number of sRNA ranging from 21 to 24 nt length expressed in the buds of the transgenic lines in October (sense in black, antisense in blue) and February (sense in grey, antisense in yellow). The flat lines shown in the NT control and in line P35S:MdDAM1#3 indicate that there were no sRNAs mapping to the T-DNA cassette. The sRNA abundance values indicated for each line is the mean from three independent biological replicates.