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. 2020 Jul 7;11:1003. doi: 10.3389/fpls.2020.01003

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Copyright © 2020 Moser, Asquini, Miolli, Weigl, Hanke, Flachowsky and Si-Ammour

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of MdDAM4 in buds of the MdDAM1 transgenic lines and during dormancy. The relative expression values of MdDAM4 in buds of the different transgenic lines MdDAM1 lines and the NT control in October (blue bars) and February (green bars) are indicated. Significance levels (Student’s t-test) relative to the NT control expressed as P values (two-tailed) were as follows: *P < 0.05 and ****P < 0.005 (A). The expression of MdDAM4 in buds of ‘Dorsett Golden’ (orange bars), ‘Anna’ (blue bars) and ‘Golden Delicious’ (black bars) cultivars collected at different time points from the onset of dormancy in October to bud break in March is indicated in (B). MdDAM4 relative expression compared to the housekeeping gene MdEF1 alpha was determined by qRT-PCR. Error bars represent standard deviation from three biological replicates.