Table 7.

The comparison of commonly used PD-L1 IHC assay.

Antibody clone	Manufacturer	Detection systems	Staining platform	Species	Heat-induced epitope retrieval	Binding sites of antibody
22C3,28-8,73-10	Dako	EnVision FLEX visualization system	Dako Autostainer Link 48	Rabbit	EnVision FLEX	extracellular domain of PD-L1
SP142, SP263	Ventana/Roche	OptiView detection kit	Ventana BenchMark ULTRA	Rabbit	CC1 Cell conditioning	the cytoplasmic domain at the extreme C-terminus of PD-L1
E1L3N	Cell Signaling Technology	Laboratory detection system	Laboratory detection system	Rabbit	Laboratory detection system	cytoplasmic domain of PD-L1