Skeletal muscle intracellular CaMKII, AMPKα and p62 signalling in response to incremental exercise to exhaustion in normoxia and severe hypoxia and the application of immediate ischaemic or non-ischaemic recovery. Protein expression levels of pThr287 CaMKII (A), pThr172 AMPKα (B), AMPKα total (C), pThr172 AMPKα/AMPKα total ratio (D), pSer349 p62 (E), p62 total (F), pSer349 p62/p62 total ratio (G), and association between the ratio of pThr172 AMPKα/AMPKα total and the ratio of pSer349 p62/p62 total (H). Nx; test performed in normoxia (FIO2 = 0.21, PIO2: 143 mmHg), Hyp; test performed in severe acute normobaric hypoxia (FIO2 = 0.104, PIO2: 73 mmHg); Pre, before exercise; Post, 10 s after the end of exercise with ischaemic recovery; Oc1m, 60 s after the end of exercise with ischaemic recovery; FC1m, 60 s after the end of exercise in the leg recovering without occlusion (free circulation); p62/SQSTM1 (shortened to p62). n = 11 in all conditions except for Oc1m Nx (n = 9), Post Hyp (n = 10), and Oc1m Hyp (n = 10). In (H), large symbols represent the mean of the subjects studied in each condition. The correlation coefficient and regression line have been calculated using the individual values (small white circles, n = 73). A detailed description of the experimental phases is explained in Fig. 1. The statistical analysis was performed with logarithmically transformed data for the pThr172 AMPKα/AMPKα total ratio and p62 total. The values shown are means ± standard errors and expressed in arbitrary units (a.u.). †p < 0.05 vs Pre Nx; *p < 0.05 vs Pre Hyp; #p < 0.05 vs Post Hyp; §p < 0.05 vs Oc1m Hyp.