Fig. 5.

Associations between the protein expression levels of phosphorylated CaMKII, Nrf2 and Keap1 across experimental phases. pThr²⁸⁷ CaMKII and pSer⁴⁰ Nrf2 (A) pThr²⁸⁷ CaMKII and Nrf2 total (B), pThr²⁸⁷ CaMKII and Nrf2/Keap1 ratio (C), pThr²⁸⁷ CaMKII and Keap1 (D). A description of the experimental phases is explained in Fig. 1. Nx; test performed in normoxia (F_IO₂ = 0.21, P_IO₂: 143 mmHg), Hyp; test performed in severe acute normobaric hypoxia (F_IO₂ = 0.104, P_IO₂: 73 mmHg); Pre, before exercise; Post, 10 s after the end of exercise with ischaemic recovery; Oc1m, 60 s after the end of exercise with ischaemic recovery; FC1m, 60 s after the end of exercise in the leg recovering without occlusion (free circulation). n = 11 in all conditions except for Oc1m Nx (n = 9), Post Hyp (n = 10), and Oc1m Hyp (n = 10). Large symbols: each point is representing the mean of the subjects studied in each condition. Correlation coefficients and regression lines have been calculated using the individual values (small white circles, n = 73). The values shown are means ± standard errors and expressed in arbitrary units (a.u.). Statistical significance was set at p < 0.05.