Fig. 7.

Representative immunoblot images of all proteins and their regulatory phosphorylations and the total amount of protein loaded (Reactive Brown staining) from a single participant. From top to bottom: pThr²⁸⁷ CaMKII, pThr¹⁷² AMPKα, AMPKα total, pSer³⁴⁹ p62/SQSTM1, p62/SQSTM1 total, pSer⁴⁰ Nrf2, Nrf2, Keap1, Catalase, SOD1, SOD2 and Reactive Brown (as protein loading control). Nx; test performed in normoxia (F_IO₂ = 0.21, P_IO₂: 143 mmHg), Hyp; test performed in severe acute normobaric hypoxia (F_IO₂ = 0.104, P_IO₂: 73 mmHg); Pre, before exercise; Post, 10 s after the end of exercise with ischaemic recovery; Oc1m, 60 s after the end of exercise with ischaemic recovery; FC1m, 60 s after the end of exercise without ischaemic recovery (free circulation); CT, control sample. Arrows indicate estimated molecular weights.