TABLE 1.
U. maydis strains used in this study.
| Strains | Relevant genotype/resistance | Strain collection no. (UMa) | Plasmids transformed/resistance | Manipulated locus | Progenitor (UMa) | References |
| FB1a | a1b1 (wild type) | 51 | / | / | Cross of wild type strains UM518 × UM521 | Banuett and Herskowitz (1989) |
| FB2a | a2b2 (wild type) | 52 | / | / | Cross of wild type strains UM518 × UM521 | Banuett and Herskowitz (1989) |
| AB33 | a2 PnarbW2bE1 PhleoR | 133 | pAB33 (Brachmann et al., 2001) | b | UMa52 | Brachmann et al. (2001) |
| FB6a a | a2b1 | 55 | / | / | Cross of wild type strains UM518 × UM521 | Banuett and Herskowitz (1989) |
| FB6b a | a1b2 | 56 | / | / | Cross of wild type strains UM518 × UM521 | Banuett and Herskowitz (1989) |
| FB2 lacZ-cts1 (screening progenitor) | a2b2 pep4::[Poma:lacZ:shh:cts1] NatR | 1501 | pUMa2373 / lacZ-Cts1_NatR | umag_ 04926 (pep4) (Sarkari et al., 2014) | UMa52 | This study |
| FB2CGL (screening strain) | a2b2 upp1:: [Pomagus:shh:cts1]HygR pep4::[Poma:lacZ:shh:cts1] NatR | 1502 | pUMa2374 / gus-cts1_HygR | umag_ 02178 (upp1) (Sarkari et al., 2014) | UMa1501 (Screening progenitor) | This study |
| FB1CGL (strain used for back-crossings) | a1b1 upp1:: [Pomagus:shh:cts1]HygR pep4::[Poma:lacZ:shh:cts1] NatR | 1547 | / | / | Derivative of crossing between FB1 and FB2CGL | This study |
| FB2 Guscyt | a2b2 ipS [Pomagus:shh]ipR CbxR | 1507 | pUMa2335 / gus_cbxR | cbx | UMa52 | This study |
| FB2 LacZcyt | a2b2 ipS [PomalacZ:shh]ipR CbxR | 1508 | pUMa2336 / lacZ_cbxR | cbx | 52 (FB2) | This study |
| FB2CGLmut1 | a2b2 upp1:: [Pomagus:shh:cts1]HygR pep4::[Poma:lacZ:shh:cts1] NatR + UV-induced mutations #24-8 | 1795 | / | UV mutagenized (Figure 3; Supplementary Figure S5) | UMa1502 (screening strain) | This study |
| FB2CGLmut2 | a2b2 upp1:: [Pomagus:shh:cts1]HygR pep4::[Poma:lacZ:shh:cts1] NatR + UV-induced mutations #4-13 | 1831 | / | UV mutagenized (Figure 3; Supplementary Figure S5). | UMa1502 (screening strain) | This study |
| FB2CGLmut3 | a2b2 upp1:: [Pomagus:shh:cts1]HygR pep4::[Poma:lacZ:shh:cts1] NatR + UV-induced mutations #3-10 | 1830 | / | UV mutagenized (Figure 3; Supplementary Figure S5). | UMa1502 (screening strain) | This study |
| AB33 jps1G | a2 PnarbW2bE1 PhleoR ipS[Pjps1::umag_03776:gfp]ipR CbxR | 2299 | pUMa3293 / CbxR | cbx | UMa133 | This study |
| AB33 cts1G | a2 PnarbW2bE1 PhleoR umag_10419:gfp NatR | 388 | pUMa828 (pCts1G-NatR) (Koepke et al., 2011) | umag_10419 (cts1) | UMa133 | Koepke et al. (2011) |
| AB33 jps1mC/Cts1G | a2 PnarbW2bE1 PhleoR umag_10419:gfp NatR umag_03776:mcherry HygR | 2048 | pUMa3034 / HygR | umag_ 03776 (jps1) | UMa388 | This study |
| AB33 jps1Δ | a2 PnarbW2bE1 PhleoR | 2092 | pUMa2775 / HygR | umag_ 03776 (jps1) | UMa133 | This study |
| AB33cts1Δ | a2 PnarbW2bE1 PhleoR umag_10419Δ HygR | 387 | pUMa780 (pCts1Δ-HygR) (Koepke et al., 2011) | umag_10419 (cts1) | 133 | Koepke et al. (2011) |
aFB1, FB2, FB6a, and FB6b are used as tester strains for mating and are derived from the same spore obtained after crossing of the wild type strains UM518 and UM521 (Banuett and Herskowitz, 1989).